Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. [UniProt]

The stated application concentrations are suggested starting points.Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 2293-2478 was used as the immunogen for the Ki67 antibody.

Store the Ki67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).