Recognizes a 34kDa protein (cdk1), identified as Cdk1/Cdc2/p34cdc2 (a catalytic subunit of Maturation Promoting Factor).Its epitope maps near the C-terminus of the protein and its core is thought to be LGTPNNEV (aa220-227 in murine cdc2).It shows no cross reaction with cdk2 p32.It supports the kinase activity.p34cdc2 plays a crucial role during cell division and is most active during mitosis.It is a serine/threonine kinase, which is activated by cyclin, by dephosphorylation of tyrosine residues.p34cdc2 is inactivated by a tyrosine kinase.This mAb reportedly inhibits the activation of p34cdc2 kinase by cyclins.

Optimal dilution of the CDC2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

A partial protein (the C-terminal two-thirds of Xenopus CDC2) was used as the immunogen for this CDC2 antibody.

Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).