366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.

Optimal dilution of the EGLN1 / PHD2 antibody should be determined by the researcher.

Amino acids 1-24 of human PHD2/EGLN1 were used as the immunogen for the EGLN1 antibody.

Store the EGLN1 / PHD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).