Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

The stated application concentrations are suggested starting points. Titration of the Histone H2AX antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 115-143 from the human protein was used as the immunogen for the Histone H2AX antibody.

Aliquot the Histone H2AX antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.