This gene encodes one of several forms of glutamic aciddecarboxylase, identified as a major autoantigen ininsulin-dependent diabetes. The enzyme encoded is responsible forcatalyzing the production of gamma-aminobutyric acid fromL-glutamic acid. A pathogenic role for this enzyme has beenidentified in the human pancreas since it has been identified as anautoantibody and an autoreactive T cell target in insulin-dependentdiabetes. This gene may also play a role in the stiff man syndrome.Alternative splicing results in multiple transcript variants thatencode the same protein.
The stated application concentrations are suggested starting points. Titration of the GAD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 109-138 from the human protein was used as the immunogen for the GAD2 antibody.
Aliquot the GAD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
