The protein encoded by this gene contains a RING fingermotif and acts as a transcription regulator. This protein has beenshown to interact with, and inhibit the activity of, TRPS1, atranscription suppressor of GATA-mediated transcription.Transcription repressor ZNF278/PATZ is found to interact with thisprotein, and thus reduce the enhancement of androgenreceptor-dependent transcription mediated by this protein. Studiesof the mouse and rat counterparts suggested a role of this proteinin spermatogenesis. A pseudogene of this gene is found onchromosome 1.
The stated application concentrations are suggested starting points. Titration of the RNF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 95-123 from the human protein was used as the immunogen for the RNF4 antibody.
Aliquot the RNF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
