Dystroglycan is a laminin binding component of thedystrophin-glycoprotein complex which provides a linkage betweenthe subsarcolemmal cytoskeleton and the extracellular matrix.Dystroglycan 1 is a candidate gene for the site of the mutation inautosomal recessive muscular dystrophies. The dramatic reduction ofdystroglycan 1 in Duchenne muscular dystrophy leads to a loss oflinkage between the sarcolemma and extracellular matrix, renderingmuscle fibers more susceptible to necrosis. Dystroglycan alsofunctions as dual receptor for agrin and laminin-2 in the Schwanncell membrane. The muscle and nonmuscle isoforms of dystroglycandiffer by carbohydrate moieties but not protein sequence.Alternative splicing results in multiple transcript variants allencoding the same protein.

The stated application concentrations are suggested starting points. Titration of the DAG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 718-747 from the human protein was used as the immunogen for the DAG1 antibody.

Aliquot the DAG1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.