This gene encodes a member of the NOD (nucleotide-bindingoligomerization domain) family. This member is a cytosolic protein.It contains an N-terminal caspase recruitment domain (CARD), acentrally located nucleotide-binding domain (NBD), and 10 tandemleucine-rich repeats (LRRs) in its C terminus. The CARD is involvedin apoptotic signaling, LRRs participate in protein-proteininteractions, and mutations in the NBD may affect the process ofoligomerization and subsequent function of the LRR domain. Thisprotein is an intracellular pattern-recognition receptor (PRR) thatinitiates inflammation in response to a subset of bacteria throughthe detection of bacterial diaminopimelic acid. Multiplealternatively spliced transcript variants differring in the 5" UTRhave been described, but the full-length nature of these variantshas not been determined.

The stated application concentrations are suggested starting points. Titration of the NOD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 923-951 from the human protein was used as the immunogen for the NOD1 antibody.

Aliquot the NOD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.