Nitric oxide is a reactive free radical which acts as abiologic mediator in several processes, including neurotransmissionand antimicrobial and antitumoral activities. This gene encodes anitric oxide synthase which is expressed in liver and is inducibleby a combination of lipopolysaccharide and certain cytokines. Threerelated pseudogenes are located within the Smith-Magenis syndromeregion on chromosome 17.
The stated application concentrations are suggested starting points. Titration of the NOS2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 830-860 from the human protein were used as the immunogen for the NOS2 antibody.
Aliquot the NOS2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
