This gene belongs to the TIMP gene family. The proteinsencoded by this gene family are natural inhibitors of the matrixmetalloproteinases (MMPs), a group of peptidases involved indegradation of the extracellular matrix. In addition to itsinhibitory role against most of the known MMPs, the encoded proteinis able to promote cell proliferation in a wide range of celltypes, and may also have an anti-apoptotic function. Transcriptionof this gene is highly inducible in response to many cytokines andhormones. In addition, the expression from some but not allinactive X chromosomes suggests that this gene inactivation ispolymorphic in human females. This gene is located within intron 6of the synapsin I gene and is transcribed in the oppositedirection.
The stated application concentrations are suggested starting points. Titration of the TIMP-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 157-188 from the human protein was used as the immunogen for the TIMP-1 antibody.
Aliquot the TIMP-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
