Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. DERL1 may act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. It may mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein.
The stated application concentrations are suggested starting points. Titration of the Derlin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 224-251 from the human protein was used as the immunogen for the Derlin 1 antibody.
Aliquot the Derlin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
