Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Alpha 2B adrenergic receptor subtype was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the alpha2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. Alpha 2B adrenergic receptor gene contains no introns in either its coding or untranslated sequences.

The stated application concentrations are suggested starting points. Titration of the Alpha-2B Adrenergic Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 343-369 from the human protein was used as the immunogen for the Alpha-2B Adrenergic Receptor antibody.

Aliquot the Alpha-2B Adrenergic Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.