Plks (polo-like kinases) encode serine/threonine kinases that are closelyrelated to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo-like kinases, which include Plk, Snk (for serum-inducible kinase, also designated Plk2) and Fnk (for FGF-inducible kinase, also designated Plk3 or PRK), play a role in cellproliferation. Plk protein accumulates in the cell during S and G2 phases ofthe cell cycle, and both protein content and catalytic activity peak at theonset of mitosis, followed by a rapid reduction after mitosis. Snk and Fnk areimmediate-early response genes that are first expressed during G1 phase.SNK may play a role in the division of at least some cell types, such as fibroblasts, and could function in embryogenesis, wound healing or neoplasiaSNK mRNA is rapidly induced in human lung fibroblasts upon reintroduction of serum following 36 hours of serum deprivation.

The stated application concentrations are suggested starting points. Titration of the Polo-like kinase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 375-406 from the human protein was used as the immunogen for the Polo-like kinase 2 antibody.

Aliquot the Polo-like kinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.