Ficolins are a group of proteins which consist of acollagen-like domain and a fibrinogen-like domain. In human serum,there are two types of ficolins, both of which have lectinactivity. The protein encoded by this gene is a thermolabilebeta-2-macroglycoprotein found in all human serum and is a memberof the ficolin/opsonin p35 lectin family. The protein, which wasinitially identified based on its reactivity with sera frompatients with systemic lupus erythematosus, has been shown to havea calcium-independent lectin activity. The protein can activate thecomplement pathway in association with MASPs and sMAP, therebyaiding in host defense through the activation of the lectinpathway. Alternative splicing occurs at this locus and twovariants, each encoding a distinct isoform, have been identified.
The stated application concentrations are suggested starting points. Titration of the Ficolin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 214-243 from the human protein was used as the immunogen for the Ficolin 3 antibody.
Aliquot the Ficolin 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
