The protein encoded by this gene is a brain-enrichednucleotide exchanged factor that contains an N-terminal GEF domain,2 tandem repeats of EF-hand calcium-binding motifs, and aC-terminal diacylglycerol/phorbol ester-binding domain. Thisprotein can activate small GTPases, including RAS and RAP1/RAS3.The nucleotide exchange activity of this protein can be stimulatedby calcium and diacylglycerol. Three alternatively splicedtranscript variants encoding the same protein have been found forthis gene.
The stated application concentrations are suggested starting points. Titration of the CalDAG-GEFI antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 7-34 from the human protein was used as the immunogen for the CalDAG-GEFI antibody.
Aliquot the CalDAG-GEFI antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
