The epsilon globin gene (HBE) is normally expressed in theembryonic yolk sac: two epsilon chains together with two zetachains (an alpha-like globin) constitute the embryonic hemoglobinHb Gower I; two epsilon chains together with two alpha chains formthe embryonic Hb Gower II. Both of these embryonic hemoglobins arenormally supplanted by fetal, and later, adult hemoglobin. The fivebeta-like globin genes are found within a 45 kb cluster onchromosome 11 in the following order: 5"-epsilon - G-gamma -A-gamma - delta - beta-3"
The stated application concentrations are suggested starting points. Titration of the HBE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 55-83 from the human protein was used as the immunogen for the HBE1 antibody.
Aliquot the HBE1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
