The melanin-concentrating hormone (MCH) is a cyclicneuropeptide isolated initially from salmon pituitary gland andlater from rat hypothalamus (summarized by Nahon et al., 1992[PubMed 1572663]). In mammals, MCH perikarya are confined largelyto the lateral hypothalamus and zona incerta area with extensiveneuronal projections throughout the brain, including theneurohypophysis. The anatomic distribution suggests aneurotransmitter or neuromodulator role for MCH in a broad array ofneuronal functions directed toward the regulation of goal-directedbehavior, such as food intake, and general arousal. MCH and 2 otherputative neuropeptides, NEI and NGE, are encoded by the sameprecursor and appear colocalized in nerve cells and in manyinstances within the projections. The precursor is designatedpro-melanin-concentrating hormone (PMCH).
The stated application concentrations are suggested starting points. Titration of the Pro-MCH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 94-122 from the human protein was used as the immunogen for the Pro-MCH antibody.
Aliquot the Pro-MCH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
