Lysyl hydroxylase is a membrane-bound homodimeric proteinlocalized to the cisternae of the endoplasmic reticulum. The enzyme(cofactors iron and ascorbate) catalyzes the hydroxylation of lysylresidues in collagen-like peptides. The resultant hydroxylysylgroups are attachment sites for carbohydrates in collagen and thusare critical for the stability of intermolecular crosslinks. Somepatients with Ehlers-Danlos syndrome type VI have deficiencies inlysyl hydroxylase activity.
The stated application concentrations are suggested starting points. Titration of the LH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 66-94 from the human protein were used as the immunogen for the LH1 antibody.
Aliquot the LH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
