Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Optimal dilution of the Collagen VII antibody should be determined by the researcher.

A recombinant full-length human Collagen VII protein was used as the immunogen for this Collagen VII antibody.

Store the Collagen VII antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).