Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5"monophosphoramidate (AMP-NH2), adenosine 5"monophosphomorpholidate (AMP-morpholidate) and guanosine 5"monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Can also convert adenosine 5"-O-phosphorothioate and guanosine 5"-O- phosphorothioate to the corresponding nucleoside 5"-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin- protein ligase complex.

The stated application concentrations are suggested starting points. Titration of the HINT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Recombinant human protein was used as the immunogen for the HINT1 antibody.

Aliquot the HINT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.