This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Optimal dilution of the EGFR antibody should be determined by the researcher.

A human recombinant partial protein (amino acids 344-492) was used as the immunogen for the EGFR antibody.

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).