This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface.CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Optimal dilution of the CD3e antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

A recombinant human partial protein corresponding to amino acids 23-119 from the human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).