Angiopoietin 2 and -1 are involved in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with -1. It is a naturally occurring antagonist of ANGPT1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. ANGPT1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

The stated application concentrations are suggested starting amounts.Titration of the Angiopoietin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Amino acids 454-468 (NLNGMYYPQRQNTNK-human) were used as the immunogen for this Angiopoietin 2 antibody.

After reconstitution, the Angiopoietin 2 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.