Activating transcription factor 3 is a member of themammalian activation transcription factor/cAMP responsiveelement-binding (CREB) protein family of transcription factors.Multiple transcript variants encoding two different isoforms havebeen found for this gene. The longer isoform represses rather thanactivates transcription from promoters with ATF binding elements.The shorter isoform (deltaZip2) lacks the leucine zipperprotein-dimerization motif and does not bind to DNA, and itstimulates transcription presumably by sequestering inhibitoryco-factors away from the promoter. It is possible that alternativesplicing of the ATF3 gene may be physiologically important in theregulation of target genes.

Titration of the ATF3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 140-168 from the human protein was used as the immunogen for this ATF3 antibody.

Aliquot the ATF3 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.