ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.

Titration of the ATF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 220-252 from the human protein was used as the immunogen for this ATF4 antibody.

Aliquot the ATF4 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.