Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, the protein can regulate p53-dependent cell cycle and cell death pathways.

Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Amino acids 652-669 (KVLDQYEREGFNFLAKVF-human) were used as the immunogen for this ATG7 antibody.

After reconstitution, the ATG7 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.