Axin-1 is a cytoplasmic protein which contains aregulation of G-protein signaling (RGS) domain and a dishevelledand axin (DIX) domain. The encoded protein interacts withadenomatosis polyposis coli, catenin beta-1, glycogen synthasekinase 3 beta, protein phosphate 2, and itself. This proteinfunctions as a negative regulator of the wingless-type MMTVintegration site family, member 1 (WNT) signaling pathway and caninduce apoptosis. The crystal structure of a portion of thisprotein, alone and in a complex with other proteins, has beenresolved. Mutations in this gene have been associated withhepatocellular carcinoma, hepatoblastomas, ovarian endometriodadenocarcinomas, and medullablastomas. Two transcript variantsencoding distinct isoforms have been identified for the gene.
Titration of the Axin-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 710-738 from the human protein was used as the immunogen for this Axin-1 antibody.
Aliquot the Axin-1 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.
