Btg3-associated nuclear protein controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 "Ser-15" phosphorylation and nuclear accumulation, which causes cell cycle arrest (By similarity).

Titration of the BANP antibody may be required due to differences in protocols and substrate sensitivity.

A human recombinant protein of amino acids 30-390 was used as the immunogen for the BANP antibody.

Aliquot the BANP antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.