The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of the protein that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

The stated application concentrations are suggested starting points.Titration of the beta-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Amino acids DDDIAALVIDNGSGK were used as the immunogen for this beta-Actin antibody.

After reconstitution, the beta-Actin antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.