The de novo synthesis of pyrimidine nucleotides isrequired for mammalian cells to proliferate. This gene encodes atrifunctional protein which is associated with the enzymaticactivities of the first 3 enzymes in the 6-step pathway ofpyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II),aspartate transcarbamoylase, and dihydroorotase. This protein isregulated by the mitogen-activated protein kinase (MAPK) cascade,which indicates a direct link between activation of the MAPKcascade and de novo biosynthesis of pyrimidine nucleotides.

Titration of the CAD antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 780-809 from the human protein was used as the immunogen for this CAD antibody.

Aliquot the CAD antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.