This gene encodes a common acute lymphocytic leukemiaantigen that is an important cell surface marker in the diagnosisof human acute lymphocytic leukemia (ALL). This protein is presenton leukemic cells of pre-B phenotype, which represent 85% of casesof ALL. This protein is not restricted to leukemic cells, however,and is found on a variety of normal tissues. It is a glycoproteinthat is particularly abundant in kidney, where it is present on thebrush border of proximal tubules and on glomerular epithelium. Theprotein is a neutral endopeptidase that cleaves peptides at theamino side of hydrophobic residues and inactivates several peptidehormones including glucagon, enkephalins, substance P, neurotensin,oxytocin, and bradykinin. This gene, which encodes a 100-kD type IItransmembrane glycoprotein, exists in a single copy of greater than45 kb. The 5" untranslated region of this gene is alternativelyspliced, resulting in four separate mRNA transcripts. The codingregion is not affected by alternative splicing. [provided byRefSeq].

Titration of the CD10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 272-300 from the human protein was used as the immunogen for this CD10 antibody.

Aliquot the CD10 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.