This antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.

The concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the CD147 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Human T cell line Molt-13 was used as the immunogen for this CD147 antibody.

Store the CD147 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

5A11 Antigen; 5F7; Basigin; Blood brain barrier HT7 antigen BSG; Extracellular Matrix Metalloproteinase Inducer (EMMPRIN); Leukocyte Activation Antigen M6; Neurothelin; OK Blood Group Antigen; TCSF; Tumor Cell-derived Collagenase Stimulatory Factor, CD147 antibody