Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27 (Workshop VI; Code 6T-028). CD27 is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.

Optimal dilution of the CD27 antibody should be determined by the researcher.

Sezary cells were used as the immunogen for the CD27 antibody.

Store the CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).