CD stands for cluster of differentiation; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes(helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

The stated application concentrations are suggested starting amounts.Titration of the CD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

CD4-transfected mouse T-cell hybridoma (3DT) followed by CD4+ human T-cell CEM cells was used as the immunogen for this CD4 antibody.

After reconstitution, the CD4 antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.