Recognizes a protein of 40kDa, identified as CD7, a member of the immunoglobulin gene superfamily. Its N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3"-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.

Optimal dilution of the CD7 antibody should be determined by the researcher.

Human T cells were used as the immunogen for the CD7 antibody.

Store the CD7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).