CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

Titration of the CDC25B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 165-194 from the human protein was used as the immunogen for this CDC25B antibody.

Aliquot the CDC25B antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.