Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.

Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.

A recombinant N-terminal protein fragment from human Clathrin heavy chain was used as the immunogen for the Clathrin Heavy Chain antibody.

Store the Clathrin Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).