This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

Store the Connexin 32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).