CCND1 is the regulatory component of the Cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. CyclinD-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. [UniProt]

Titration of the Cyclin D1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 68-97 from the human protein was used as the immunogen for this Cyclin D1 antibody.

Aliquot the Cyclin D1 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.