Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3" exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

The stated application concentrations are suggested starting points. Titration of the DNA Polymerase alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

A portion of amino acids 1406-1439 from human POLA1 was used as the immunogen for the DNA Polymerase alpha antibody.

Aliquot the DNA Polymerase alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.