Protein Kinase DNA-Activated catalytic subunit, also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Satoh et al.(1997) mapped the MCM4 gene to 8q11.2 by FISH. Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location. Anderson and Lees-Miller(1992) noted that DNA-PKcs had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription. Daniel et al.(1999) demonstrated that the DNA-PKcs protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.

The stated application concentrations are suggested starting amounts.Titration of the DNA-PKcs antibody may be required due to differences in protocols and secondary/substrate sensitivity.

An amino acid sequence from the N-terminus of human PRKDC (AKNAEMHKNKLQYFMEQFY) was used as the immunogen for this DNA-PKcs antibody.

After reconstitution, the DNA-PKcs antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.