Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated "Lys-36" of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]

The stated application concentrations are suggested starting points. Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Recombinant human Dnmt3a was used as the immunogen for the Dnmt3a antibody.

Aliquot the Dnmt3a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.