Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4% vs. 94.7%.

The concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.

A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.

Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).