This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

The concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

The immunizing protein for this antibody was recombinant human EGFR.

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; Urogastrone; wa2; Wa5, EGFR antibody, ERBB1 antibody