The epidermal growth factor receptor (EGFR; ErbB1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB1), HER2/c-neu (ErbB2), Her 3 (ErbB3) and Her 4 (ErbB4). It exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to overexpression or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiform.

The stated application concentrations are suggested starting points.Titration of the EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Human partial recombinant protein (AA 25-346) was used as the immunogen for this EGFR antibody.

After reconstitution, the EGFR antibody can be stored for up to one month at4oC.For long-term, aliquot and store at -20oC.Avoid repeated freezing and thawing.