Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. [UniProt]

The concentration stated for each application is a general starting point.Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

Recombinant human protein was used as the immunogen for this EGFR antibody.

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).