Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Optimal dilution of the Filaggrin antibody should be determined by the researcher.

Amino acids 198-288 of human FLG were used as the immunogen for this Filaggrin antibody.

Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).