The protein encoded by this gene is a type I transmembraneglycoprotein which shows homology to the pMEL17 precursor, amelanocyte-specific protein. GPNMB shows expression in the lowlymetastatic human melanoma cell lines and xenografts but does notshow expression in the highly metastatic cell lines. GPNMB may beinvolved in growth delay and reduction of metastatic potential. Twotranscript variants encoding different isoforms have been found forthis gene.
Titration of the GPNMB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 541-569 from the human protein was used as the immunogen for this GPNMB antibody.
Aliquot the GPNMB antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.
