Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody.

Aliquot the H4 antibody and store frozen at -20oC or colder.Avoid repeated freeze-thaw cycles.