The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5"- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3". The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5" untranslated regions and the introns, but they differ significantly over the 3" untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some non-deletion alpha thalassemias have also been reported.

Optimal dilution of the Hemoglobin antibody should be determined by the researcher.

Amino acids V2-R142 from the human alpha subunit were used as the immunogen for the Hemoglobin antibody.

After reconstitution, the Hemoglobin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.